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- The resultant msAb is bivalent with respect to the target tissue and DOTA
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For example, bscAbs are produced by joining two single-chain Fv fragments via a glycine-serine linker using recombinant methods. The V light-chain (VL) and V heavy-chain (VH) domains of two antibodies of interest are isolated using standard PCR methods. The VL and VH cDNA's obtained from each hybridoma are then joined to form a single-chain fragment in a two-step fusion PCR. The first PCR step introduces the (Gly4-Ser1)3 (SEQ ID NO: 8) linker, and the second step joins the VL. and VH amplicons. Each single chain molecule is then cloned into a bacterial expression vector. Following amplification, one of the single-chain molecules is excised and sub-cloned into theother vector, containing the second single-chain molecule of interest. The resulting bscAb fragment is subcloned into an eukaryotic expression vector. Functional protein expression can be obtained by transfecting the vector into chinese hamster ovary cells. Bi-specific fusion proteins are prepared in a similar manner. Bi-specific single-chain antibodies and bi-specific fusion proteins are included within the scope of the present invention.
Non-radioactive metals are also of use in the invention. An example is gadolinium, which is a useful metal for magnetic resonance imaging. Similarly to the radiometals, a solution of non-radioactive gadolinium in acid is neutralized to pH 4-6 using excess ammonium acetate. The gadolinium added to the DOTA-peptide at an equimolar ratio of gadolinium to the DOTA moieties present. After a one-hour labeling time at 45° C, the added gadolinium is substantially bound to the DOTA-peptide.
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For mice 4 and 5 the last immunization is carried out intravenously, with hLL2-DOTA-Y, 100 μg in PBS
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Culture media supernatant of aminopterin-resistant hybrids are screened by ELISA for antibodies to DOTA-Yttrium using the same assay as used for the sera. The hybrids culture supernatants are tested undiluted on plates coated with BSA-DOTA-Yttrium, and positive hybrids are checked for absence of binding to hLL2, in a similar ELISA assay using plates coated with hLL2. Hybrids found to secrete in their culture supernatants an IgG binding to BSA-DOTA-Yttrium but not to hLL2 are selected for cloning.
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A total of 1800 clones were screened by ELISA for reactivity with DOTA-conjugated hIgG and hIgG alone
The msAbs of the present invention include, but are not limited to, IgG×IgG, IgG×F(ab′)2, IgG×Fab′, IgG×scFv, F(ab′)2×F(ab′)2, Fab′×F(ab′)2, Fab′×Fab′, Fab′×scFv and scFv×scFv bi-specific monoclonal antibodies (bsmAbs). Also, species such as scFv×IgG×scFv and Fab′×IgG×Fab′, scFv×F(ab′)2×scFv and Fab′×F(ab′)2×Fab′ are included. Advantageously, site-specific attachment sites on the IgG or F(ab′)2 of one or both monoclonal antibodies (mAbs) can be utilized, such as an engineered carbohydrate or an engineered or liberated free thiol group.
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NcR athymic nude mice are implanted with cells of the human colonic tumor cell line, termed GW-39. When tumors have reached an appropriate size (>100 mm3) agents are injected. The I-125-radioiodinated msAb is given first, and at different later times the In-111-IMP-236 is administered. The latter radiolabeled peptide is also given alone to a control group of animals. Doses given to each animal are 1/5×10−10 moles of I-125-hMN-14×mLG1 (8 uCi of I-125 per animal), and 1/5×10−11 moles of In-111-IMP-236. At specified times post-injection of the labeled IMP-236 animals are sacrificed and major internal tissues are collected and quantified for both I-125 and In-111 radioactivity. The data is summarized in the Tables below.
The LG1 antibody was shown to bind equivalently well to the uncomplexed DOTA-hLL2 as to DOTA-hLL2 complexed with the metals gadolinium, gallium, indium, lead, lutetium and yttrium. These metals represent a diverse group in terms of binding properties, valency, coordination number and stereochemistry.
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The present invention relates to an antibody or antibody fragment that binds to 1,4,7,10-tetrazacyclododecane-N,N′,N″,N′″-tetraacetic acid (DOTA),which is bound to an alkyl-amino group through one of its pendant carboxyl groups. Moreover, the invention relates to a metal complex of DOTA wherein the metal bound to DOTA (http://zbsmarket.ru/free/?key=1329) binds with essentially equivalent affinity to peptide-DOTA-metal complexes of gallium, indium, gadolinium, yttrium, lutetium, and lead.
Mouse Anti-DOTA Recombinant Antibody (clone 2D12.5
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On SE-HPLC with radiomatic detection, the In-111 radiolabeled peptide elutes near 14/8 minutes, and when mixed with an excess of the msAb hMN-14×LG1 and re-applied to SE-HPLC the radioactivity peak is shifted to a retention time of near 10/4 minutes. These tests show the purity of the In-111-IMP-236 and the retained ability of the In-111-labeled peptide to be bound by the LG1-containing msAb.
The present invention can be used in Boron Neutron Capture Therapy (BNCT) protocols. BNCT is a binary system designed to deliver ionizing radiation to tumor cells by neutron irradiation of tumor-localized boron-10 atoms. BNCT is based on the nuclear reaction which occurs when a stable isotope, isotopically enriched B-10 (present in 19/8% natural abundance), is irradiated with thermal neutrons to produce an alpha particle and a Li-7 nucleus. These particles have a path length of about one cell diameter, resulting in high linear energy transfer. Just a few of the short-range 1/7 MeV alpha particles produced in this nuclear reaction are sufficient to target the cell nucleus and destroy it. Success with BNCT of cancer requires methods for localizing a high concentration of boron-10 at tumor sites, while leaving non-target organs essentially boron-free.
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Multi-specific antibodies (msAbs) offer the possibility of improved efficacy in the delivery of radionuclides using antibody targeting. Radionuclide therapy can be more efficacious when the radionuclide is attached to a moiety that is bivalent toward the pretargeted msAb.
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A typical peptide containing more than one DOTA (read more here) per backbone is Ac-Phe-Lys(DOTA)-Tyr-LyS(DOTA-NH2 (SEQ ID NO: 5). It has been radiolabeled with 111 In and shown to bind strongly to the LG1 mAb. It will be appreciated that numerous modifications to the size and other properties of this construct are facile to one skilled in the art of peptide synthesis.
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When preparing msAbs chemically, IgG-1×IgG-2-type conjugates can be prepared (where IgG-1 is the targeting mAb and IgG-2 is the LG1 mAb), as can IgG-1×F(ab′)2-2; F(ab′)2-1×IgG-2; F(ab′)2-1×F(ab′)2-2; IgG-1×Fab′-2; F(ab′)2-1×Fab′-2; Fab′-1×Fab′-2; Fab′-1×F(ab′)2-2; and Fab′-1×IgG-2. Sub-fragments smaller than Fab's can also be used, including sFvs, as can alternatively prepared fragments such as Fabs. For instance, a mAb directed towards carcinoembryonic antigen (CEA), anti-CEA F(ab′)2, having an engineered light-chain carbohydrate can be oxidized and converted using a hydrazide-maleimide cross-linker to a derivatized anti-CEA F(ab′)2 having at least one pendant maleimide group per each light chain. This species is coupled to LG1-Fab′-SH at a 1:2 molar ratio or greater, such that an anti-DOTA-Fab′×anti-CEA-F(ab′)2-anti-DOTA-Fab′ conjugate is produced. The resultant msAb is bivalent with respect to the target tissue and DOTA (http://zbsmarket.ru/free/?key=1601). Quadromas can also be made that secrete IgG-1×IgG-2 msAbs.
For this, a DOTA conjugate of humanized LL2, with an average DOTA-to-hLL2 molar substitution ratio of 2/8-to-1 is used. A 49 mM solution of yttrium chloride (YCl3) in 50 mM hydrochloric acid is prepared using yttrium chloride hexahydrate (of 99/999% purity). An aliquot of this YCl3 solution (0/05 mL) is buffered with 0/3 mL of 0/25 M ammonium acetate pH 5/4. The DOTA conjugate of hLL2 (2 mL; 16/7 mg) is mixed with 0/238 mL of the buffered yttrium (approximately 15-fold molar excess) and heated at 45° C. for 30 minutes. This is followed by an additional 15-minutes of heating, subsequent to a DTPA-quench, using 0/248 mL of 0/1 M DTPA pH 6/7. The solution is cooled, and purified on two successive spin-columns (“centrifuged size-exclusion chromatography”) of Sephadex G50/80 in 0/1 M sodium acetate pH 6/5. The final eluate is sterile-filtered. Matrix-assisted laser desorption ionization [MALDI] mass spectral analyses of both the uncomplexed hLL2-DOTA conjugate and yttrium-chelated conjugate gives a mass difference of 651 Daltons (151,837 for hLL2-DOTA versus 152,488 for hLL2-DOTA-yttrium), with an error rate of 0/2% in this mass range.
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The present inventive antibodies or antibody fragments can be used in a method of photodynamic diagnosis or therapy. In the diagnostic method, a diagnostic agent is injected, for example, systemically, and laser-induced fluorescence can be used by endoscopes to detect sites of cancer which have accreted the light-activated agent. For example, this has been applied to fluorescence bronchoscopic disclosure of early lung tumors (Doiron et al, Chest 76:32, 1979), incorporated herein by reference. In another example, the inventive antibodies and antibody fragments can be used in single photon emission. For example, a Tc-99m-labeled diagnostic agent can be administered to the subject following administration of the msAbs. The subject is then scanned with a gamma camera which produces single-photon emission computed tomographic images and defines the lesion or tumor site.
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The msAb of the kit may be monoclonal or polyclonal in nature. Furthermore, both the target arm and the capture arm can be monoclonal.
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A functional bscAb can be produced by the coexpression in E. coli of two “cross-over” scFv fragments in which the VL and VH domains for the two fragments are present on different polypeptide chains. The VL and VH domains of two antibodies of interest are isolated using standard PCR methods. The cDNA's are then ligated into a bacterial expression vector such that C-terminus of the VL domain of the first antibody of interest is ligated via a linker to the N-terminus of the VH domain of the second antibody. Similarly, the C-terminus of the VL domain of the second antibody of interest is ligated via a linker to the N-terminus of the VH domain of the first antibody.
The LG1 Ab of the current invention is monoclonal in nature. Also, the LG1 Ab may be chimeric, humanized, human or deimmunized.
Amino acid sequence is shown as one-letter codes. The amino acid residues form CDRs (according to Kabat numbering scheme) are underlined and indicated as L1-3.
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Additionally, transgenic plants facilitate long term storage of bscAb and fusion proteins. Functionally active scFv proteins have been extracted from tobacco leaves after a week of storage at room temperature. Similarly, transgenic tobacco seeds stored for 1 year at room temperature show no loss of scFv protein or its antigen binding activity.
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A rapid clearance profile via the urine is preferred and an intact complex should ideally be excreted. Thus, the molecule that comprises the radiolabeled hapten must remain relatively hydrophilic. There should be no appreciable retention of the radiolabeled hapten in any non-targeted organ. The hapten should be capable of being labeled with metal at high specific activity, so that most hapten is associated with a metal (nuclide) atom. The chemical linkages binding the recognition and chelate-nuclide units to the backbone, and the backbone itself, must not be hydrolytically or enzymatically cleaved for the duration of the protocol. There needs to be an adequate separation of the recognition units of the hapten with >10 ångströms producing optimal recognition and binding.
As used herein, the term subject means any animal, especially a mammal, including a non-human primate or a human. The terms “subject,” “organism,” and “patient” may be used interchangeably.
A variety of bi-specific fusion proteins can be produced using molecular engineering. In one form, the bi-specific fusion protein is monovalent, consisting of, for example, a scFv with a single binding site for one antigen and a Fab fragment with a single binding site for a second antigen. In another form, the bi-specific fusion protein is divalent, consisting of, for example, an IgG with two binding sites for one antigen and two scFv with two binding sites for a second antigen.
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The hMN14 Fab′-SH fragment is prepared by reducing the F(ab′)2 with 1 mM dithiothreitol (DTT). The pure Fab′ is obtained by diafiltration into pH 5/3 acetate buffer containing 0/5 mM EDTA. LG1-Fab is derivatized with excess sulfo-SMCC to obtain an activated LG1 Fab-maleimide, containing maleimide groups for linking to the free thiol groups group on the hMN14 Fab′ fragment. Following removal of excess sulfo-SMCC by diafiltration into pH 5/3 acetate buffer containing 0/5 mM EDTA, LG1 Fab-maleimide is mixed with an approximately equal amount of hMN14 Fab′-SH to produce the multi-specific conjugate. The conjugation reaction is quenched by the addition of cysteine to a final concentration of 1 mM. The desired product, hMN14-Fab′×LG1-Fab msAb, molecular weight 100 kD, is obtained pure using Superdex 200 column chromatography.
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After 10 days the clones are tested for IgG binding to BSA-DOTA-Yttrium
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The terms antibody and antibody fragments are used interchangeably herein. Furthermore, as used herein, the term antibody may also be used to refer to bi-specific antibodies (bisAbs) or multi-specific antibodies (msAbs). The terms bisAb and msAb are used interchangeably herein. Antibody fragments are antigen binding portions of an antibody, such as F(ab′)2, F(ab)2, Fab′, Fab, and the like. The antibody fragments bind to the same antigen that is recognized by the intact antibody. For example, an anti (discover here)-CD22 monoclonal antibody fragment binds to an epitope of CD22.
The IMP 236 peptide from example 11 is dissolved in 0/25 M NH4OAc/10% HPCD buffer at concentrations of 9, 18, 35, 70 and 140 ug/mL. The solutions are sterile-filtered through a 0/22 um Millex GV filter in one mL aliquots into acid washed lyophilization vials. The filled vials were frozen immediately on dry ice after filling and lyophilized. When the lyophilization cycle was complete the vials were septum-sealed under vacuum and crimp-sealed upon removal from the lyophilizer.
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Are you interested in any of these changes? Have you been playing Conan Exiles recently? Let us know your thoughts in the comments section below.
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Funcom released a hotfix update yesterday for Conan Exiles. The update is mostly focused on bug fixes and AI improvement. However, some balancing changes have also been made. The update also enabled the previously announced anti-undermesh feature for the ‘Exiled Lands’ map. You can learn more about it over here.
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Many of the difficulties associated with generating monoclonal antibodies by B-cell immortalization can be overcome by engineering and expressing antibody fragments in E. coli, using phage display. To ensure the recovery of high affinity monoclonal antibodies a combinatorial immunoglobulin library must contain a large repertoire size. A typical strategy utilizes mRNA obtained from lymphocytes or spleen cells of immunized mice to synthesize cDNA using reverse transcriptase. The heavy- and light-chain genes are amplified separately by PCR and ligated into phage cloning vectors. Two different libraries are produced, one containing the heavy-chain genes and one containing the light-chain genes. Phage DNA is islolated from each library, and the heavy- and light-chain sequences are ligated together and packaged to form a combinatorial library. Each phage contains a random pair of heavy- and light-chain cDNAs and upon infection of E. coli directs the expression of the antibody chains in infected cells. To identify an antibody that recognizes the antigen of interest, the phage library is plated, and the antibody molecules present in the plaques are transferred to filters. The filters are incubated with radioactively labeled antigen and then washed to remove excess unbound ligand. A radioactive spot on the autoradiogram identifies a plaque that contains an antibody that binds the antigen.
Therefore, as is known in the art for any DNA sequence determined by this automated approach, any nucleotide sequence determined herein may contain some errors. Nucleotide sequences determined by automation are typically at least about 90% identical, more typically at least about 95% to at least about 99/9% identical to the actual nucleotide sequence of the sequenced DNA molecule. The actual sequence can be more precisely determined by other approaches including manual DNA sequencing methods well known in the art.